METHODOLOGY FOR DETERMINING AND ANALYZIS OF THE FEATURES OF THE DISTRIBUTION OF THE MASS OF COMPONENTS IN SYSTEMS OF THE "ENVIRONMENT - SUBSTANCE" TYPE
DOI:
https://doi.org/10.32636/agroscience.2026-(5)-2-8Keywords:
samples, method, methodology, mass, content, ratio, compositionAbstract
Development of methodological techniques for analyzing the features of changes in the equilibrium state of the mass of organic and inorganic substances in systems of the "environment - substance" type under the action of exo- and endogenous factors, which provide an objective assessment of the features of the distribution of the components of these systems and the establishment of parameters of the harmful and/or protective effects of individual factors on the homeostasis of their mass. To solve the tasks set, the gravimetric method was applied and a number of physicochemical properties of the components were used, namely: the ability of organic substances to burn at low and high temperatures; the ability of inorganic substances to dissolve in distilled water, hydrochloric acid and its mixture with nitric acid (aqua regia or water of the tsars "V.C."); the ability of centrifugal force to divide the sample into liquid and sediment; the ability of electrolyte molecules to dissociate into ions. The evaluation of the determined indicators was carried out by a specially introduced calculation formula (Δх = х0 ± хi) of the delta of changes in the mass of components under the action of exo- and endogenous factors. The applied techniques and methods of a simple methodology for analyzing the results of the research provide the determination of absolute and relative mass indicators of flammable (FL) and heat-resistant (TS) organic (OR) and inorganic (NR) substances of mineralized ash (MA) of powdered samples of dry residue (SZ), dissolved and undissolved components of MA with distilled water and concentrated acids, making significant adjustments to the identification and specification of harmful and/or protective effects. The obtained indicators allow not only to analyze the features of the distribution of components in systems of the "environment - substance" type, but also to substantiate their relationship between pairs (H2O:SZ; H2O:MZ; H2O:LZ; H2O:TS; SZ:TS; MZ:TS; LZ:TS; SZ:LZ; SZ:MZ; SZ:MZ; MZ:LZ).
References
Afanasiieva L. P., Kalynovskyi H. М. Permeability of heavy metals across the placental barrier of the cow. Visnyk Sumskoho natsionalnoho universytetu, 2007. No 8 (19). P. 5–8.
Law of Ukraine "On the Protection of Animals from Cruelty". Information of the Verkhovna Rada of Ukraine. 2006. No 27. URL: https://zakon.rada.gov.ua/lows/show/3447-15#Text(23.08.2024)
Kalynovskyi H. M., Yevtukh L. H., Shnaider V. L. et al. Penetration across the placental barrier for Cadmium (C) and Lead (P) during pregnancy in cows and during foaling in mares. Naukovyi visnyk LNUVMB imeni S. H. Hzhytskoho, Seriia: Veterynarni nauky. 2019. Vol. 21, No 93. P. 74–87. doi:10.32718/nvlvet9314.
Maksymiuk H.V., Vorobets D.Z., Lapovets L Ye., Sanahurskyi D.I., Maksymiuk V.М. Method for determining the concentration of free and bound ions in aqueous extract fractions (AEF) of biological material samples. Patent of Ukraine for a utility model № 69773, МПК G01N 21/00; Publ. 10.05.2012., Biul. No 9.
Maksymiuk H. V., Vorobets Z. D., Lapovets L. Ye., Fafula R. V., Sedilo H. M, Maksymiuk V. M., Sharan М. М. Method for assessing changes in ion homeostasis in cervical mucus samples under the influence of endo- and exogenous factors. Patent of Ukraine for a utility model № 119753, МПК G01N 33/48; G01N 21/00; G01N 21/41; Publ. 10.10. 2017, Biul. No 19.
Maksymiuk H. V., Vorobets M. Z.., Stadnytska O. I., Sedilo H. M, Maksymiuk V. М. Imbalance of mass and concentration of components of secretions and fluids of the genital organs due to the action of harmful factors: metod. rek. Lviv-Obroshyne, 2023. 40 p.
Maksymiuk H. V., Vorobets M. Z.., Stadnytska O. I., Sedilo H. M, Maksymiuk V. М. Homeostasis: action and consequence (Methods, methodology, research methodology). Obroshyne, 2025. 180 p.
Malynin O. O., Kutsan O. T., Litarova M. V., Puzanov F. К. Method for quantitative determination of inorganic elements in biological substrates using X-ray fluorescence analysis. Patent of Ukraine for a utility model № 42619, МПК G01N 23/00; Publ. 10.07.2009 р., Biul. No 13.
Agbugba, L. C., Oyewunmi, A. O., Ogundumade, T. P., & Leigh, O. O.. Investigation of vaginal mucus parameters: Development of models for staging the oestrous cycle of the Bunaji cow. Reproduction in domestic animals // Zuchthygiene. 2020. Vol. 55 (9), P. 1044–1053. https://doi.org/10.1111/rda.13694.
Chen, Z., Myers R., Wei T. [et al.] Placental transfer and concentrations of cadmium, mercury, lead and selenium in mothers, newborns, and young children // Journal of Exposure Science and Environmental Epidemiology. 2014. Vol. 24. № 5. P. 537–544. doi: 10.1038/jes.2014.26.
Directive of European Parliamentand od the Council of 22 September 2010 on the protection of animals used for scientific, 22.09. 2010/63/EU. URL:https://eur-lex.europa.eu/eli/dir/2010/63/oj (last accessed: 26.08.2024).
Essawi WM, Mostafa DIA and El Shorbagy AIA. Comparison between Biochemical Analysis of Cattle Amniotic Fluid and Maternal Serum Components during Pregnancy // World Vet. J.2020. 10 (1): 67-73. DOI: https://dx.doi.org/10.36380/scil.2020.wvj9.
Fontes, P. L. P., Oosthuizen, N., Ciriaco, F. M. [et al.]. Impact of fetal vs. maternal contributions of Bos indicus and Bos taurus genetics on embryonic and fetal development1 // Journal of animal science. 2019. 97(4), 1645–1655. https://doi.org/10.1093/jas/skz044.
Kipper, M., Hoque A. M. W., Raqib R. Accumulation of cadmium in human placenta interacts with transport of micronutrients to the foetus // Toxically Lett. 2010. Vol. 192. № 2. P. 162–168. doi: 10.1016/j.toxlet.2009.10.018.
Lin, C. M., Doyle, P., Wang, D. Does prenatal cadmium exposure effect foetal and child growth? // Occupational and Environmental Medicine. 2011. Vol. 68. № 9. P. 641–646. doi: 10.4172/2161-0509.1000204.
Oleksy-frenzel J. et.al. “Application of ion-chromatography for the determination of the organic group parameters AOCL, AOBr and AOI in water” Fresenius J. Anal. Chem., 2000, 366. p. 89 − 94.
Sharaf, M. A., Illman, D. M. Cytmometrics. New York : John Wiley & sons. 1989. 269 P.
Siregar, T. N., Armansyah, T., Panjaitan, B. et al. Changes in cervical mucus as an indicator of fertility in ache cattle // Advances in Animal and Veterinary Sciences. 2019. Vol. 7 (4), P. 306‒314. http://dx.doi.org/10.17582/journal.aavs/2019/7.4.306.31.
Suliburska, J., Kocyłowski, R., Komorowicz, I., Grzesiak, M., Bogdański, P., & Barałkiewicz, D. Concentrations of Mineral in Amniotic Fluid and Their Relations to Selected Maternal and Fetal Parameters. // Biological trace element research. 2016. 172(1), Р. 37–45. https://doi.org/10.1007/s12011-015-0557-3
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Copyright (c) 2026 Ганна МАКСИМЮК, Ольга СТАДНИЦЬКА, Григорій СЕДІЛО, Зіновій ВОРОБЕЦЬ, Василь МАКСИМ’ЮК (Автор)

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