22CSN3- HinfI AND BLG- Hae III POLYMORPHISM IN THE DISAPPEARING BROWN CARPATIAN CATTLE BREED

Abstract

The article presents the results of the study of structural genes in animals of the Brown Carpathian breed of cows, which are associated with milk productivity: CSN3 (kappa-casein), BLG (beta-lactoglobulin). These genes control the processes of milk production in many mammals, including cows. A total of 2 gene polymorphisms (CSN3 and BLG) were investigated. The study used 30 samples of DNA isolated from the venous blood of Brown Carpathian cows using the «DNA Sorb-B» kit (AmpliSens). Genotyping was performed using polymerase chain reaction (PCR-RFLP) polymorphism analysis of restriction fragment lengths. As a result of the research, it was found that polymorphism of CSN3 and BLG genes is represented by alleles CSN3^А, CSN3^В and BLG^A, BLG^В and, respectively, genotypes CSN3^АА, CSN3^АВ, CSN3^ВВ and BLG^АА, BLG^АВ, BLG^ВВ. For the CSN3 gene, an amplified fragment with a size of 273 bp. treated with HinfI restriction enzyme. A high frequency of the CSN3^A allele was established at 0.635 and a slightly lower frequency of the CSN3^B allele at 0.365. When studying the BLG gene, the amplification product (247 bp) was treated with the restriction enzyme HaeIII. It was found that allele B (0.59) and heterozygous genotype BLG^AB (0.44) were more common. The results of the study are valuable in connection with the sharp reduction in the number of small and aboriginal populations and the threat of extinction of the own genetic resources of agricultural species