Effectiveness of the usage of an experimental preparation for animals based on oil solutions of nanodiamonds of detonation synthesis, modified with β-carotine
DOI:
https://doi.org/10.32636/01308521.2022-(72)-2-11Keywords:
rabbits, heifers, detonation synthesis Nanodiamonds, beta-carotene, Tetravit, non-specific resistance, biochemical indicators, albumins, globulinsAbstract
The harmlessness of the experimental preparation for animal organism that based on oil solutions of nanodiamonds of detonation synthesis modified with β-carotene. Efficiency of the use of experimental preparation was determined in the conditions of physiology court of Institute of Animal Sciences of NAAS on crawls and SF of experience economy of "Gontarivka" of the Kharkiv area on the heifers of the Ukrainian black-white suckling breed of accidental age.
The better digestibility of this preparation administered intramuscularly in experimental rabbits was confirmed. These rabbits’ growth, weight and body length have increased in comparison with the peers who received the drug subcutaneously. The experimental rabbits’ motor activity did not differ within group selection and the pathological conditions were not detected.
A probable positive effect on increasing the level of β-carotene in experimental heifers’ blood serum was fixed. Biochemical analysis of the experimental heifers’ blood serum showed that the carotene content in the blood of heifers in the control group (saline solution) almost was not changing during the experiment. After injection of the experimental heifers of Tetravit the level of β-carotene in their blood serum increased by 102.8 mg% (20.9 %), while the level of carotene in the blood serum of heifers that were injected with the experimental preparation increased by 301.0 mg% (61.2 %). The indicators of the content of β-carotene increased due to the fact that the animals that were injected with Tetravit have already been restored the content of vitamin A in the body and beta-carotene, which came with feed, remained in the body in an unchanged form, while Beta-carotene was transformed into vitamin A in the animals that were not injected with Tetravit, so we see the difference in the content of β-carotene in the blood serum of the 1st group (control group) and the 2nd research group (Tetravit). A significant increase in the level of beta-carotene in the 3rd research group, that was administered the experimental drug, can be explained by the fact that the nanodiamonds contained in the preparation drug have increased the assimilation of β-carotene from the feed received by the body significantly. The rest of the studied biochemical indicators of the animals’ blood serum of all groups were also within the reference values and changed in different degrees after the injection of the preparations. It was found that after calving, the cows of the experimental group had an increased level of carotene in the blood (1.045±0.031 mg%), 0.252 mg% higher than before calving. A month after re-introduction of the drug and two months after the beginning of lactation, the level of carotene in the blood decreased slightly (0.092 mg%). Accordingly, the content of vitamin A also decreased.
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