PHYSIOLOGICAL INDICATORS OF THE BLOOD OF CROSSBRED HEIFERS AND COWS OF THE UKRAINIAN BLACK-SPOTTED DAIRY X HOLSTEIN BREED OF DIFFERENT TYPES OF CONSTITUTION
DOI:
https://doi.org/10.32636/agroscience.2023-(2)-3-5Keywords:
physiological indices of blood, heifers, type of constitutionAbstract
All physiological processes in the animal body are accompanied by corresponding changes in the blood. The state and intensity of metabolic processes in the animal's body can be determined by changes in the hemogram in different age periods. Erythrocytes take part in the water and salt balance of the animal body, they swell in the venous blood and give excess fluid to the arterial blood. This feature of them is especially important for the processes of breathing and sweating.
Blood serum protein is the main indicator of protein biosynthesis in the animal body. The analysis of the obtained data shows that according to the content of total protein in the blood serum, heifers at 6, 12, 18 months of age, heifers at 8-9 months of gestation and first-born cows at 2-3 months of lactation of the research group exceeded the control analogues respectively by 2.48; 10.24; 6.90; 10.42 and 10.12%. The control group (n=10) included animals with a low physiological selection index, and the experimental group (n=10) with a high one.
On average, over the entire period of the experiment, the advantage of animals of the experimental group over the control group was 6.36%. Therefore, according to the content of total protein in blood serum, animals of the high-enzyme type (experimental group) significantly prevailed over the analogues of the low-enzyme type (control group). This suggests that the level of protein metabolism in the animals of the experimental group was more intense than in the control counterparts.
An important component of protein metabolism in the body of farm animals are trans-amination enzymes – aspartate and alanine aminotransferases (AST and ALT). The study of catalase activity in the blood of experimental heifers at 3, 6, 12, 18 months of age, heifers at 8-9 months of gestation and cows at 2-3 months of lactation showed that the animals of the experimental group exceeded the control females respectively by 11.52; 11.02; 11.34; 10.84; 15.37; 5.81%. On average, over the entire period of the experiment, the animals of the experimental group prevailed over the control group by 10.54%.
In general, according to the biochemical parameters of the blood in the postnatal ontogeny, the animals of the experimental group significantly exceeded the control counterparts. This indicates that the level of metabolic processes in the body of the animals of the experimental group was much more intense than in the control counterparts
References
Breeding situation in DPDG "Oleksandrivske" for breeding Ukrainian red-spotted and black-spotted dairy breeds and ways to improve it. / G. S Kovalenko and others. Rozvedennia i henetyka tvaryn. 2016. Vol. 51. P. 69–73.
Buchko O. M. Free radical processes in the cattle lets for the actions of enzymatic additives. Anim. Biol. 2013. No 15(1). P. 27–33.
Buchko O. M., Maksimovic I. Y. Link glutathione antioxidant protection of holstein cattle under the influence of humic additives. Nature Anim. Biol. 2011. No 13. P. 60–65.
Burtis C. A., Ashwood E. R., Bruns D. E. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 4th Edition. MO: Elsevier Saunders. 2015. 2448 p. DOI: 10.1373/clinchem.2005.062638
Chernyak N. G., Goncharuk O. P. Estimation of the first-born cows of the Ukrainian black-and-white dairy breed by type of body structure in the breeding farm LLC "Sukholiske". Rozvedennia i henetyka tvaryn. 2017. Vol. 41. P. 276–280.
Fеdаk V. D., Fеdаk N. М. Physiological and biochemical bases of the formation of productive traits of cattle. Teoretychni ta praktychni rekomendatsii. 2011. Оbrоshyn. 19 p.
Fіlоnеnkо М. А., Sаifеtdіnоvа H. А. Experimental modeling of dysmorphic damage of erythrocytes in Holstein cows. Ukrainskyi biokhimichnyi zhurnal. 2015. № 4 (30). P. 27-33.
Guder W. G., Schmidt Z. U. Erythrocytes cell heterogeneity. The distribution of pyruvatekinase and phosphoenolpyruvate carboxykinase (GTP) in the blood of holstein cattle fed microbial protein additives. J. Physiol. Chem. 2005. Vol. 357. P. 1793-1800.
Huston P. Building a blood system types in animal science during the 21st century. Canadian animal sci. journ. 2017. No 3. P. 88-95.
Hrybаn V. H., Yefіmоv О. S., Rаkytianskyi V. М. The use of preparations of protein nature in combination with trace elements for the correction of metabolism in cows. Naukovyi visnyk NAU. К. 2018. Issue 78. P. 64–66.
Kaminskyi V. F., Gadzalo J. M., Bashchenko M. I. Modern tendencies and directions of development of organic animal husbandry. Naukovi osnovy vyrobnytstva orhanichnoi produktsii tvarynnytstva v Ukraini. 2016. No 15. P 359–364.
Khalak V. I. Biochemical indicators of serum and their associative relationship with fattening and meat qualities of young heifers of different intrabreed differentiation by body mass index. Rozvedennia i henetyka tvaryn. 2018. Vol. 41. P. 78–89.
Khmelnychyi L. M., Khmelnychyi S. L. Population and genetic parameters of linear conformation traits cows firstborn ukrainian black-and-white dairy breed. Rozvedennia i henetyka tvaryn. 2019. Vol. 58. P. 67–69.
Khmelnychyi L. M., Vechorka V. V. Viability of cows of Ukrainian black-spotted and red-spotted dairy breeds depending on the assessment of linear features of the exterior. Visnyk Sumskoho natsionalnoho ahrarnoho universytetu. Seriia «Tvarynnytstvo. 2017. Vol. 7 (33). P. 48–58.
Khmelnychyi L. M., Vechorka V. V., Khmelnychyi S. L. Features of the exterior type of dairy cattle of different origins and the relative variability of linear characteristics with milking of Holstein cows. Rozvedennia i henetyka tvaryn. 2018. Vol. 56. P. 77–83.
Коzhеmіakin Yu. М., Fіlоnеnkо М. А., Sаifеtdіnоvа H.А. Scientific and practical recommendations for keeping farm animals and working with them. К.: Avitsenna. 2008. 155 p.
Lysеnkо М. V., Bоikо V. І., Zаmаzіi М. D. Anatomy and physiologyof farm animals: Pidruchnyk. К.: Libra. 2019. 415 p.
Mazzachi B. C., Peake M. J., Ehrhardt V. S. Reference range and method of comparison studies for enzymatic and creatinine assays in blood plasma and serum. Clinical laboratory. 2000. No 46(1–2). P. 53–5.
McCunn M., Karlin A. Nonblood fluid resuscitation. Anesth. clinics of North Amer. 2019. No 1. P. 107-123.
Nаumеnkо V. V., Dеmchеnkо V. Yu. The influence of microelements on indicators of gas-energy and protein-mineral exchanges in Holstein cattle in the conditions of the Steppe of Ukraine. Naukovyi visnyk NAU. К. 2015. Issue 61. P. 75–86.
Nаumеnkо V. V., Dеmchеnkо V. Yu., Physiology of farm animals: Pidruchnyk. К.: Prosvita. 2014. 289 p.
Omelkovych S. P., Lisogurska D. V. Characteristics of economically useful qualities of cows of Ukrainian black-and-white dairy breed of different genotypes and their compliance with the parameters of dairy animals. Zbirnyk naukovykh prats Vinnytskoho natsionalnoho ahrarnoho universytetu. 2022. Vol. 5 (67). P. 135–140.
Оvsіenkо А. І., Кulyk О. К., Stаsiuk V. D. Effective use of proteins in modern feeding of Holstein cows. Mizhvidomchyi tematychnyi naukovyi zbirnyk Kormy i kormovyrobnytstvo. К.: Ahrarna nauka. 2014. № 54. P. 173-185.
Pаnіn L. Е., Usynіn І. F. Changes in the activity of key enzymes of protein metabolism in subpopulations of hepatocytes, non-parenchymal liver cells and blood of cattle. Naukovyi visnyk NAU. К. 2016. Issue 75. P. 15–24.
Pelekhatyi L. M., Kochuk-Yashchenko O. S. Estimation of dairy productivity of cows by exterior. Tvarynnytstvo Ukrainy. 2014. № 11. P. 5-9.
Pochukalin A. E, Rizun O. V, Priyma S. V The level of basic and additional breeding traits in highly productive herds of Ukraine. Naukovyi visnyk «Askaniia-Nova. 2018. Vol. 11. P. 122–130.
Polupan Yu. P., Melnyk Yu. F., Biryukova O.D. Influence of genetic factors on the productivity of cows. Rozvedennia i henetyka tvaryn. 2019. Vol. 58. P. 41–51.
Polupan Yu. P. Prospects for pedigree improvement of dairy farming. Ahrobiz. sohodni. 2012. № 20 (243). P. 98–103.
Physiology of farm animals / еditor А. Y. Маzurkеvych, V. І. Каrpоvskyi. Pidruchnyk. Vinnytsia: NK. 2018. 345 p.
Schmucker B. L., Mooney A. L., Jones J. C. Stereological analysis of membrane fine structure in the oxygenated erythrocytes of cattle. J. Cell Biol. 2017. Vol. 78. P. 319-337.
Shаmеlаshvіlі К. L., Sеrhіienkо М. V., Hоrіlа N. І. Blood superoxide dismutase and catalase: enzyme activity under conditions of oxidative stress. Naukovyi zhurnal «ScienceRise». 2015. № 5(10). P. 11-15.
Shcherbatyi P. V., Bodnar V. P., Kropyvka Yu. G. Dynamics of live weight growth and exterior-constitutional features of cows of the Ukrainian black- and-white dairy breed of different types of constitution. Naukovyi visnyk Lvivskoho natsionalnoho uversytetu veterynarnoi medytsyny ta biotekhnolohii imeni S. Z. Gzhytskoho. 2016. Vol. 18. № 2 (67). P. 281–286.
Shirakami Y. L., Clugston R. D., Blaner. W. S. Hepatic metabolism of retinoids and disease associations. Biochim. Biophys. Acta. 2021. No 82. P. 124–136.
Stepchenko L. M., Skoryk T. R. Condition of erythrocyte antioxidant function in cattle by the actions of humic substances. Technical bulletin of Scientific Institute of Animal Biology and State research control institute of veterinary preparations and feed additives. 2016. Vol. 7. № 3, 4. С. 137–143.
Stepchenko L. M., Skoryk M. V. System status of erythrocyte antioxidant protection of cattle under the influence of humic substance. Scientific Bulletin of Lviv National University of Veterinary Medicine and Biotechnology named after S. Z. Gzhytsky. 2018. No 7(3–4). P. 137–143.
Тоpurіia L. Yu., Тоpurіia H. М. The influence of drugs of natural origin on the metabolism of substances, immune status and erythropoiesis in cattle. Naukovyi visnyk NAU. К. 2021. Issue 95. P. 41–56.
Visser S. A. Effect of some chemical substances on mitochondiral respiration and oxidative phosphorylation in blood erythrocytes of cattle. Sci. Total. Environ. 2008. Vol. 62. P. 347-354.
Wilson J. W. Cellular structure in relation to function of erythrocytes, approaches to the quantitative description of the liver function. Int. Journ. Biol. Sci. 2009. No 14. P. 175-197.
Zhu H. N., Jia Z. S., Misra H. L. Oxidative stress and redox signaling mechanisms of erythrocytes by some liver disease, updated experimental and clinical evidence. J. Dig. Dis. 2019. No 13(3). P. 133–142.
Zhurakivska O. Ya., Bodnarchuk Yu. V., Pertsovych V. M. Morphofunctional changes in the blood erythrocytes of 24-month-old holstein heifers in the early stages of experimental diabetes mellitus development. Word of medicine and biology. 2018. No 3(65). P. 152–156
Downloads
Published
Issue
Section
License
Copyright (c) 2023 Vasyl FEDAK, Mykhailo POLULIKH, Olga STADNYTSKA, Vasyl BRATIUK, Volodymyr DUTKA, Roman OSEREDCHUK (Author)

This work is licensed under a Creative Commons Attribution 4.0 International License.
