Unesterified fatty acids level in abdomen tissues, and bees’ productive traits at feeding various quantity of linseed oil
DOI:
https://doi.org/10.32636/01308521.2020-(67)-2-17Keywords:
honey bees, feed additive, linseed oil, fatty acids, queen bee’s reproductive ability, honey productivity of beesAbstract
The research goal is to establish a connection between the composition of unesterified fatty acids in abdomen tissues and honey bees productive traits at various quantity of linseed oil in a feed additive.
The experimental studies were conducted in spring-summer period on clinically healthy Carpathian breed honey bees. There were three groups of bee families (3 bee colonies in each group), selected by analogues principle. Bee colonies of the control group were receiving a feed additive, consisting of 100 g of defatted flour of natural soybeans and 100 g of sugar syrup (the ratio of sugar to water 1:1) weekly for 36 days. Bee colonies of І and ІІ experimental groups additionally to this supplement were having linseed oil in the amount of 10 and 20 g/bee colony/week, respectively. While performing the research, queen bee’s reproductive capacity and workers honey productivity were controlled. Bee tissue samples were selected for laboratory trials after feeding completion. The content of unesterified fatty acids was determined by gas-liquid chromatography method in honey bee abdominal tissues.
It is established, that as a result of adding to the feed supplement linseed oil in the amount of 10 and 20 g, consisting of defatted soybean flour and sugar syrup, it dose-dependently increases the content of saturated, monounsaturated, and, particularly, polyunsaturated fatty acids in fatty acids composition of total lipids, as well as in composition of unesterified fatty acids. Feeding of the food supplement enriched with linseed oil causes decrease in concentration of unesterified saturated with an even and odd number of Carbon atoms in a link, monounsaturated fatty acids of ω-7 and ω-9 families, and polyunsaturated fatty acids of ω-6 family in honey bee abdominal tissues of І and ІІ experimental groups. Herewith, in the mentioned bees’ abdomen tissues, the content of unesterified fatty acids of ω-3 family increases, as well as the ratio of unesterified polyunsaturated fatty acids concentration of the ω-3 family to polyunsaturated fatty acids of the ω-6 family. As a final result, the queen bees of the mentioned groups increase oviposition, and the working bees increase the honey productivity.
References
1. Кількісні хроматографічні методи визначення окремих ліпідів і жирних кислот у біологічному матеріалі : метод. посіб. / Й. Ф. Рівіс та ін. 2-ге вид., уточн. та доп. Львів : СПОЛОМ, 2017. 160 с.
2. Лаврехин Ф. А., Панкова С. В. Биология медоносной пчелы. 3-е изд., перераб. и доп. Москва : Колос, 1983. 303 с.
3. Abedi E., Sahari M. A. Long-chain polyunsaturated fatty acid sources and evaluation of their nutritional and functional properties. Food Sci. Nutr. 2014. Vol. 2, Is. 5. P. 443–463.
4. AL-Kahtani S. N. Fatty acids and B vitamins contents in honey bee collected pollen in relation to botanical origin. Scientific Journal of King Faisal University (Basic and Applied Sciences). 2017. Vol. 18, № 2. Р. 41–48.
5. Antibacterial immune competence of honey bees (Apis mellifera) is adapted to different life stages and environmental risks / H. Gӓtschenberger et al. PLoS One. 2013. Vol. 8, Is. 6. DOI: 10.1371/journal.pone.0066415.
6. A pollen fatty acid enhances learning and survival in bumblebees / F. Muth et al. Behavioral Ecology. 2018. Vol. 29, Is. 6. P. 1371–1379.
7. Arien Y., Dag A., Shafir S. Omega-6:3 ratio more than absolute lipid level in diet affects associative learning in honey bees. Front. Psychol. 2018. Vol. 9. Р. 1–8.
8. Arrese E. L., Soulages J. L. Insect fat body: energy, metabolism, and regulation. Annu. Rev. Entomol. 2010. Vol. 55. P. 207–225.
9. Brodschneider R., Crailsheim K. Nutrition and health in honey bees. Apidologie. 2010. Vol. 41, Is. 3. P. 278–294.
10. Cohen A. C. Insect diets: science and technology. Boca Raton, Florida, USA : CRC Press, 2004. 324 p.
11. Comparative transcriptome analysis on the synthesis pathway of honey bee (Apis mellifera) mandibular gland secretions / Y. Wu et al. Scientific Reports. 2017. Vol. 7 (1), 4530. DOI: 10.1038/s41598-017-04879-z.
12. Couture P., Hulbert A. J. Membrane fatty acid composition is related to body mass in mammals. The Journal of Membrane Biology. 1995. Vol. 148, Is. 1. Р. 27–39.
13. Effect of industry on the content of fatty acids in the tissues of the honey-bee head / V. Y. Vishchur et al. Ukrainian Journal of Ecology. 2019. Vol. 9, № 3. P. 174–179.
14. Fat metabolism in insects / L. E. Canavoso et al. Annual Review of Nutrition. 2001. Vol. 21. P. 23–46.
15. Fatty acid and proximate composition of bee bread / M. Kaplan et al. Food Technol. Biotechnol. 2016. Vol. 54, № 4. P. 497–504.
16. Giri S., Dillon M. E. Seasonal and altitudial variation in fatty acid composition of native bees. UW NPS Annu. Rep. 2012. Vol. 35, Is. 1. P. 23–30.
17. Gooley Z. C., Gooley A. C. Temporal variation of fatty acid composition and pesticides in honeybees Apis mellifera and beebread. bioRxiv. 2019. DOI: https://doi.org/10.1101/572149.
18. Honey bees dance faster for pollen that complements colony essential fatty acid deficiency / S. Zarchin et al. Behavioral Ecology and Sociobiology. 2017. Vol. 71, Is. 12. DOI: https://doi.org/10.1007/s00265-017-2394-1.
19. Hulbert A. J. Metabolism and longevity: Is there a role for membrane fatty acids? Integrative and Comparative Biology. 2010. Vol. 50, Is. 5. P. 808–817.
20. Hulbert A. J., Abbott S. K. Nutritional ecology of essential fatty acids: an evolutionary perspective. Australian Journal of Zoology. 2011. Vol. 59, № 6. P. 369–379.
21. Hulbert A. J., Kelly M. A., Abbott S. K. Polyunsaturated fats, membrane lipids and animal longevity. Journal of Comparative Physiology B: biochemical, systemic, and environmental physiology. 2014. Vol. 184, Is. 2. P. 149–166.
22. Influence of pollen nutrition on honey bee health: do pollen quality and diversity matter? / G. Di Pasquale et al. PLoS One. 2013. Vol. 8, Is. 8. e72016. DOI: 10.1371/journal.pone.0072016.
23. Lipid storage and mobilization in insects: current status and future directions / E. L. Arrese et al. Insect Biochemistry and Molecular Biology. 2001. Vol. 31. Р. 7–17.
24. Loidl A., Crailsheim K. Free fatty acids digested from pollen and triolein in the honeybee (Apis mellifera carnica Pollmann) midgut. Journal of Comparative Physiology B. 2001. Vol. 171, Is. 4. P. 313–319.
25. Manning R. Artificial feeding of honeybees based on an understanding of nutritional principles. Animal Production Science. 2016. Vol. 58, № 4. P. 689–703.
26. Nutritional aspects of honey bee-collected pollen and constraints on colony development in the eastern Mediterranean / D. Avni et al. Journal of Insect Physiology. 2014. Vol. 69. P. 65–73.
27. Nutritional effect of alpha-linolenic acid on honey bee colony development (Apis mellifera L.) / L. Ma et al. Journal of Apicultural Science. 2015. Vol. 59, № 2. Р. 63–72.
28. Omega-3 deficiency impairs honey bee learning / Y. Arien et al. Proc. Natl. Acad. Sci. USA. 2015. Vol. 112, № 51. Р. 15761–15766.
29. Rabiee F., Modaresi M., Gheisari A. The effect to various oleic acid levels on reproductive parameters in queen bee. Der Pharmacia Lettre. 2015. Vol. 7, Is. 12. Р. 326–331.
30. Standard methods for Apis mellifera beeswax research / L. Svečnjak et al. Journal of Apicultural Research. 2019. Vol. 58, Is. 2. P. 1–108.
31. Ziegler R., van Antwerpen R. Lipid uptake by insect oocytes. Insect Biochem. Mol. Biol. 2006. Vol. 36, Is. 4. P. 264–272.
Downloads
Published
Issue
Section
License
Copyright (c) 2020 І. І. САРАНЧУК (Автор)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
