Enzymatic activity of the rumen microbiota and intensity of growth of young ewes under the alimentary action of prebiotic supplement ISGD
DOI:
https://doi.org/10.32636/01308521.2023-(73)-2-9Keywords:
prebiotic, young sheeps, feeding, ruminal metabolism, growth intensity of animalsAbstract
The research was carried out in the conditions of the sheep farm of the State Enterprise “Experimental Farm “Hrusiatychi” (Lviv region, Zhydachiv district, Hrusiatychi village) and the department of small animal husbandry of the Institute of Agriculture of the Carpathian region of the National Academy of Agrarian Sciences of Ukraine. For this purpose, 4 groups of 11-month-old young ewes with a live weight of 38.0‒38.2 kg, 5 animals each, were formed. The duration of the experiment – 2 months (February ‒ March).
The main ration of the control group young ewes consisted of 1.1 kg of meadow cereal-herb hay and 0.5 kg of standard compound feed. Animals of the first, second, and third experimental groups were additionally fed ISGD prebiotic as part of compound feed in amounts, respectively: 1.0; 1.4 and 1.8 % of its mass.
At the end of the experimental period, after the morning feeding, from 3 animals of each investigited groups, with the help of oro-esophageal probe,ruminal fluid was collected, in which the enzymatic activity of the microbiota was determined. In order to determine the intensity of growth of the young ewes, their monthly weighing was carried out. The live weight of the animals was determined at the beginning and at the end of the experimental period.
It has been established that the use of the prebiotic in the feed of animals in doses of 1.0; 1.4 and 1.8 % of the weight of concentrate feed increases the enzymatic activity of amylolytic, proteolytic and cellulolytic microorganisms of the rumen by 13.7‒120.5; 1.9‒19.0 and 5.2‒10.5 %, compared to animals that did not receive such a supplement as part of the diet. The use of ISGD prebiotic in the indicated doses as part of the combined fodder for young ewes during the experimental period increases the average daily increase in the live weight of animals by 1.3‒1.6 times. The most pronounced stimulatory effect on the digestive activity of the symbiotic microbiota of the rumen and the intensity of animal growth is revealed by 1.4 % of the prebiotic ISGD from the mass of compound feed.
References
1. Вовк С. О., Польовий І. В. Науково-практичні аспекти використання пробіотиків у годівлі жуйних тварин. Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С. З. Ґжицького. 2020. Т. 22, № 92. С. 9–14. DOI: 10.32718/nvlvet-a9202.
2. Лабораторні методи дослідженнь у біології, тваринництві та ветеринарній медицині : довідник / В. В. Влізло та ін. ; за ред. В. В. Влізла. Львів : Сполом, 2012. 764 с.
3. Bezpalko А. V. Effect of feed additive Actisaf Cz 47 on dairy productivity of high-yielding cows, compared to baker's dry yeast. Bull. Zhytomyr National Agro-Ecological University. 2012. 2 (33): P. 104–106 (in Ukrainian).
4. Casper Calf starter containing a blend of essential oils and prebiotics affects the growth performance of Holstein calves / H. Liu et al. J. Dairy Sci. 2020. Vol. 103. P. 2315–2323.
5. Direkvandi E., Mohammadabadi T., Salem A. Oral administration of lactate producing bacteria alone or combined with Saccharomyces cerevisiae and Megasphaera elsdenii on performance of fattening lambs. J. Appl. 2020. 48. P. 235–243. DOI: 10.1080/09712119.2020.1773830.
6. Effects of Saccharomyces cerevisiae supplementation and anhydrous ammonia treatment of wheat straw on in-situ degradability, rumen fermentation and growth performance of yearling lambs / M. Cömert et al. Asian-Australasian Journal of Animal. 2015. 28: P. 639–646. DOI: 10.5713/ajas.14.0757.
7. Effects of yeasts on rumen bacterial flora, abnormal metabolites, and blood gas in sheep with induced subacute ruminal acidosis / G. Han et al. Animal feed science and technology. 2021. Vol. 280. P. 115–142.
8. Effects of supplementation of active dried yeast and malate during sub-acute ruminal acidosis on rumen fermentation, microbial population, selected blood metabolites, and milk production in dairy cows / M. Malekkhahi et al. Anim. Feed Sci. Technol. 2016. Vol. 213. P. 29–4.
9. Effect of Saccharomyces cerevisiae and Mannan-Oligosaccharides on Daily Weight Gain and Health of Pre-Weaned Holstein Calves in Chile / P. Melendez et al. American Journal of Animal and Veterinary Sci. 2018. 13 (1). P. 1–6. DOI: 10.3844/ajavsp.2018.1.6.
10. Effect of β-1.3/1.6-Dglucan on meat performance and non-specific humoral defense mechanisms in lambs / S. Milewski
et al. Med. Vet. 2007. 63(3). P. 360–363. cabdirect.org/cabdirect/abstract/20073053051.
11. Effect of prebiotic and probiotic supplementation on growth performance and body measurement in pre-ruminant Surti buffalo calves / P. Ratre et al. J. Pharm. Innov. 2019. 8. P. 265–269.
12. Effects of active dried Saccharomyces cerevisiae on ruminal fermentation and bacterial community during the short-term ruminal acidosis challenge model in Holstein calves / Y. Watanabe et al. J. Dairy Sci. 2019. 102(7). P. 6518–6531. DOI: 10.3168/jds.2018-15871.
13. Effects of Saccharomyces cerevisiae fermentation products on the microbial community throughout the gastrointestinal tract of calves / J. Xiao et al. Animal. 2019. 9. P. 4–11. DOI: 10.3390/ani9010004.
14. Effects of single or combined supplementation of probiotics and prebiotics on ruminal fermentation, ruminal bacteria and total tract digestion in lambs / O. Zapata et al. Small Rum. Res. 2021. 204. P. 106–138. DOI: 10.1016/j.smallrumres.2021.106538.
15. Effects of feeding various dosages of Saccharomyces cerevisiae fermentation product in transition dairy cows / E. M. Zaworski et al. J. Dairy Sci. 2014. 97(5). P. 3081–3098. DOI: 10.3168/jds.2013-7692.
16. Ghosh S., Mehla R. Influence of dietary supplementation of prebiotics (mannanoligosaccharide) on the performance of crossbred calves. Trop. Anim. Health Prod. 2012. Vol. 44. P. 617–622. DOI: 10.1007/s11250-011-9944-8.
17. Graham M., Ronald Ross W., Collier J. Pre- and probiotic supplementation in ruminant livestock production. Bioactive Foods in Health Promotion. 2016. Vol. 2. P. 25–36.
18. Invited Review: Strategic use of microbial-based probiotics and prebiotics in dairy calf rearing / L. R. Cangiano et al. Appl. Anim. 2020. 36. P. 630 – 651.
19. Leicester H. C., Robinson P. H., Erasmus L. J. Effects of two yeast based direct fed microbials on performance of high producing dairy cows. Anim. Feed. Sci. Technol. 2016. Vol. 215. P. 58–72.
20. Markowiak P., Śliżewska K. The role of probiotics, prebiotics and synbiotics in animal nutrition. Gut pathogens. 2018. Vol. 10. P. 2–20. DOI: 10.1186/s13099-018-0250-0.
21. Meta-analysis of the influence of Saccharomyces cerevisiae supplementation on ruminal parameters and milk production of ruminants / M. Desnoyer et al. J. Dairy. 2009. 92 (4). P. 1620–1632. DOI: 10.3168/jds.2008-1414.
22. Mohammed S., Mahmood F., Abas E. A review on effects of yeast (Saccharomyces cerevisiae) as feed additives in ruminants performance. J. Entomol. Zool. Stud. 2018. 6. P. 629–635.
23. Prebiotics and probiotics in feed and animal health / R. C. Gupta et al. Nutraceuticals in Veterinary: Springer AG; Cham, Switzerland. 2019. P. 261–285.
24. Radzikowski D. Effect of probiotics, prebiotics and synbiotics on the productivity and health of dairy cows and calves. World Scientific News. 2017. 78. P. 193–198. DOI: 10.1264/jsme2.ME14176.
25. Relevance of probiotic, prebiotic and synbiotic supplementations on hemato-biochemical parameters, metabolic hormones, biometric measurements and carcass characteristics of sub-tropical Noemi lambs / S. F. Mehanna et al. Int. J. Anim. Res. 2017. Vol. 1. P. 10 – 19. DOI: 10.28933/ijar-2017-09-3001.
26. Robinson P. H., Erasmus L. J. Effects of analyzable diet components on responses of lactating dairy cows to Saccharomyces cerevisiae based yeast products: A systematic review of the literature. Anim. Feed Sci. Technol. 2009. 149(3–4). P. 185–198. DOI: 10.1016/-j.anifeedsci.2008.10.003.
27. Selected Alternative Feed Additives Used to Manipulate the Rumen Microbiome / M. Michalak et al. Animals. 2021. 11. P. 1542–1549. DOI: 10.3390/ani11061542.
28. Sethy K., Dhaigude V., Duibedi B. Prebiotics in animal feeding. The Pharma Innovation J. 2017. 6(11). P. 482–486.
29. Singh A., Kerketta S., Yogi R. Prebiotics – The New Feed Supplement for Dairy Calf. International Journal of Livestock Research. 2017. 7(8). P. 1–17. DOI: 10.5455/ijlr.20170610051314.
30. Stover M. G., Watson R. R., Collier R. J. Pre- and Probiotic Supplementation in Ruminant Livestock Production. Probiotics, Prebiotics, and Synbiotics: Bioactive Foods in Health Promotion. 2016. P. 25–36. DOI: 10.1016/B978-0-12-802189-7.00002-2.
31. The effects of supplementation of yeast (Saccharomyces cerevisiae) and postbiotic from Lactobacillus acidophilus on the health and growth performance of young Jersey heifer calves / M. Thorsteinsson et al. J. Anim. and Feed Sci. 2020. 29(3). P. 224–233. DOI: 10.22358/JAFS/127447/2020.
32. Use of live yeast and mannan-oligosacharides in grain-based diets for cattle: Ruminal parameters, nutrient digestibility, and inflammatory response / T. García-Díaz et al. PLoS ONE. 2018. P. 13–19. DOI: 10.1371/journal.pone.0207127.
33. Uyeno Y., Shigemori S., Shimosato T. Effect of probiotics, prebiotics on cattle health and productivity. Microbes Environ. 2015. 30(2). P. 126–132. DOI: 10.1264/jsme2.ME14176.
34. Villot C. Saccharomyces cerevisiae boulardii CNCM I-1079 affects health, growth, and fecal microbiota in milk-fed veal calves. J. Dairy Sci. 2019. 102. P. 7011–7025. DOI: 10.3168/jds.2018-16149.
35. Zhang C., Zhan J., Yu Z. Effects of supplementation with Saccharomyces cerevisiae products on dairy calves: A meta-analysis. J. Dairy Sci. 2022. 105. P. 7386–7398. DOI: 10.3168/jds.2021-21519.
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