Методика визначення співвідношень маси води та білкових і небілкових речовин матково-вагінального слизу

Ганна МАКСИМЮК; Ольга СТАДНИЦЬКА; Григорій СЕДІЛО; Василь МАКСИМ’ЮК; Ірина ЯРЕМЧУК

doi:10.32636/01308521.2024-(76)-1-10

Methodology for determining mass ratios of water, protein and non-protein substancesof the uterine-vaginal mucus

Authors

Hanna MAKSYMIUK Lviv National Medical University named after Danylo Halytskyi Author https://orcid.org/0000-0001-9561-2381
Olha STADNYTSKA Institute of Agriculture of the Carpathian region of the NAAS Author https://orcid.org/0000-0001-6574-4068
Hryhorii SEDILO Institute of Agriculture of the Carpathian region Author https://orcid.org/0000-0001-6037-9014
Vasyl MAKSYMIUK Institute of Agriculture of the Carpathian region Author https://orcid.org/0000-0001-6280-8214
Iryna YAREMCHUK Institute of Animal Biology of the NAAS Author https://orcid.org/0000-0001-8787-2132

DOI:

https://doi.org/10.32636/01308521.2024-(76)-1-10

Keywords:

cows, uterine-vaginal mucus, mass of mucus substances and their ratio

Abstract

The results of methodological development show: different extraction times (5 min → 24 → 48 → 72 h) of the studied samples with distilled water; their 24-hour dissolution with hydrochloric acid and a mixture of “royal vodka” acids and the gravimetric method (weighing, evaporation, centrifugation, combustion) divide the composition of the samples into: absorbed and bound water; extracted with water or eliminated in water (free, weakly, strongly bound); not extracted with water; dissolved and undissolved by mineral acids and acid-resistant substances. Experimenters of various kinds (physiologists, biochemists, biophysicists) can effectively apply methods tested and implemented in laboratory work to study the dynamics, distribution, and equilibrium state of the mass of components in systems of the “environment (water) ‒ substance” type. This makes it possible to objectively evaluate changes in the physical and chemical state of substances under the harmful effects of exogenous and endogenous factors.

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Published

2024-09-30

Issue

Vol. 76 No. 1 (2024)

Section

STOCKBREEDING

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This work is licensed under a Creative Commons Attribution 4.0 International License.

How to Cite

Hanna MAKSYMIUK, Olha STADNYTSKA, Hryhorii SEDILO, Vasyl MAKSYMIUK, & Iryna YAREMCHUK. (2024). Methodology for determining mass ratios of water, protein and non-protein substancesof the uterine-vaginal mucus. Foothill and Mountain Agriculture and Stockbreeding, 76(1), 99-108. https://doi.org/10.32636/01308521.2024-(76)-1-10

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